In vitro evaluation of protiens anti-denaturation and antioxidant activities of commercially available Matricaria chamomilla L.

Abstract

This study extensively investigated the in vitro therapeutic potential of Matricaria chamomilla (chamomile) extracts prepared using ethanol, acetone, and water, bay evaluating their anti- denaturation and antioxidant activities. The anti-denaturation activity, employing bovine serum albumin, determined the aqueous extract to be highly active, exhibiting 75.93% inhibition of protein denaturation at its maximum concentration, significantly more than diclofenac sodium (21.81%) and Vitamin C (41.15%). At low dilutions , chamomile extracts still showed positive inhibitory activities, demonstrating their continuing effectiveness. The DPPH assay indicated the aqueous extract as a powerful scavenger (67.62% inhibition) and the ethanolic extract next at a close second (61.38%), with pro-oxidant activity in acetonic and aqueous extracts at dilutions that were very low. The FRAP assay indicated the greatest ferric reducing antioxidant power for the ethanolic extract (92.66% inhibition).These findings collectively emphasize the tremendous potential of chamomile as a natural source of anti-inflammatory and antioxidant agents worthy of further work on characterization of compounds, in-vivo studies, finding of molecular mechanisms, and comprehensive toxicity and pharmacokinetic studies